qPCR Template Qualification using NanoDrop One and Qubit 4
In modern genomic workflows, there are multiple points where quantification is critical. Together the NanoDrop One Spectrophotometer and Qubit 4 Fluorometer provide a complete solution for your nucleic acid quantification and qualification needs. In addition to concentration, combining these two techniques gives researchers an accurate picture of their sample, detects degraded RNA before RT-qPCR reactions, ensures enough template is used in TaqMan assays, and that the template is free of contaminants such as phenol that can potentially harm sensitive polymerases. When used together, these two techniques allow researchers to obtain a clearer picture of sample quality than either technique alone. Experimental success depends in part on accurate evaluation of the sample used.