How to use Invitrogen No-Stain Protein Labeling Reagent and the iBright Imaging System for fast, easy, and accurate quantitative western blotting

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Quantitative western blotting is commonly performed using housekeeping proteins as sample loading controls however, as housekeeping proteins can fluctuate as a result of experimental conditions, total protein normalization (TPN) is a more accurate method to use to normalize western blotting data. Invitrogen No-Stain Protein Labeling Reagent offers the benefits of TPN and also allows you to use your gel of choice. This video demonstrates how fast and it is to use Invitrogen No-Stain Protein Labeling Reagent after transferring your gel to a membrane, and following western blotting, to use the iBright Imaging System, with onboard normalizing software, to capture and quantitate western blotting data using total protein normalization.

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