A step-by-step workflow for a knock-in experiment in an adherent cell line

In this tutorial video, we demonstrate the process of conducting a beta actin gene knock-in in a HEK293 adherent cell line. Learn how to design the experiment; prepare both cells and reagents; conduct post-transfection assessment; and validate the knock-in for further experimentation. For additional support designing CRISPR gene editing experiments, visit https://thermofisher.com/geneediting.