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How to Normalize cDNA Concentrations - Ask TaqMan Episode 15
Doug provides a helpful reference document which explains in detail how researchers can quantitatively validate their choice of a control gene.
3:15
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Rare Mutation Detection with Digital PCR - ASHG 2014
Product manager Dr. Iain Russell speaks about the new QuantStudio 3D rare mutation analysis solution. The solution includes a subset of wet lab–validated TaqMan SNP Genotyping Assays to detect & quantify the most common cancer-related mutations.
4:32
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Choosing the Right TaqMan Assay - Ask TaqMan Ep20
In this video, learn how to make an informed choice about which of the millions of Thermo Fishers' assays are most appropriate for experimental needs.
3:55
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Fixing Software Setup Mistakes in Real-Time PCR (SetpOnePlus) - AskTaqMan - Ep 10
Thermo Fisher Scientific Sr. Field Application Specialist Doug Rains offers advice for fixing common software set-up mistakes when performing Real-Time PCR.
3:44
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How to Use the hPSC Scorecard Analysis Software
This video will show you how to analyze your TaqMan hPSC Scorecard Panel data using the hPSC Scorecard Analysis Software.
3:07
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What Is The Thermo Fisher Cloud? - Ask TaqMan #33
Sometimes it can be a real headache, with data stored in various places, and everyone using a different program for analysis.
4:01
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Choosing The Right Master Mix For Your qPCR Experiment - Ask TaqMan #32
Choosing the right master mix is an important step when performing your experiment because you want to make sure it is completely optimized for success.
3:51
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TaqMan Assay Formats Explained - Ask TaqMan #29
Well, the answer to this will depend on your application, the number of genes and samples you want to study, and the number of replicates. Let's break it down.
5:49
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Detecting Copy Number Variation (CNV) with Digital PCR - Ask TaqMan #28
Copy Number Variations, commonly referred to as CNV's, can result in having either too many or too few of a particular loci.
4:12
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Absolute Quantification of mRNAs - Ask TaqMan #26
Relative quantitation is the most common application with real-time PCR, but sometimes fold change data is just not enough.
4:53
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Homozygous vs. Heterozygous Samples in qPCR - Ask TaqMan #23
Did you know that TaqMan assays can also be used for SNP Genotyping? While the assays use the same robust TaqMan chemistry, they contain two probes, and thus analysis is different than when using a Gene Expression assay.
4:36
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Getting Reliable Data in qPCR - Ask TaqMan #22
At the end of the day you want to be able to write up your results and know the changes you see are real -- and not the result of handling or pipeting error.
3:56
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