A step-by-step workflow for a knock-out experiment in an adherent cell line

In this tutorial video, we demonstrate the process of conducting a HPRT gene knock-out experiment in a HEK293 adherent cell line. Learn how to design the experiment; prepare both cells and reagents; conduct post-transfection assessment; perform genomic cleavage detection; and isolate and clonally expand the culture for further experimentation. For additional support designing CRISPR gene editing experiments, visit https://thermofisher.com/geneediting.