A step-by-step workflow for a knock-out experiment in iPSCs

In this tutorial video, we demonstrate the process of conducting a gene knock-out experiment in induced pluripotent stem cells. Learn how to design the experiment; prepare both cells and reagents; conduct post-transfection assessment; perform genomic cleavage detection; isolate and clonally expand the culture; and validate the resultant stem cells. For additional support designing CRISPR gene editing experiments, visit https://thermofisher.com/geneediting.