How to prepare Essential 8 Medium for pluripotent stem cell culture

This video will show you how to prepare complete Essential 8 Medium for the consistent, robust, cost-effective feeder-free culture of PSCs. [AUDIO TRANSCRIPT ] This video will show you how to prepare complete Essential 8 Medium for the feeder-free culture of PSCs Essential 8 Medium is a fully defined, feeder-free medium formulated for the growth and expansion of human pluripotent stem cells. It was originally developed as E8 Medium in the laboratory of James Thomson, and validated by Cellular Dynamics International. Thaw Essential 8 Supplement (50X) at 2--8°C overnight. Do not thaw at 37°C. To prepare 500 mL of complete Essential 8 Medium, aseptically mix the following components: DMEM/F-12 (HAM) 1:1 490 mL Essential 8 Supplement (50X) 10 mL Complete Essential 8 Medium can be stored at 2--8°C for up to 2 weeks. Before using, warm the complete amount of medium required for that day at room temperature until it's no longer cool to the touch. Don't warm the medium at 37°C.