Qubit© 2.0 Fluorometer in Action

To Learn More visit, http://www.lifetechnologies.com/qubit The Qubit® 2.0 Fluorometer quantitates DNA, RNA, and protein with unprecedented accuracy, sensitivity, and simplicity. It is designed for molecular biology labs that work with: • Precious samples that are rare or difficult to process • Applications requiring precise measurement such as real-time PCR The Qubit® 2.0 Fluorometer utilizes specifically designed fluorometric technology using Molecular Probes® dyes. These fluorescent dyes emit signals ONLY when bound to specific target molecules, even in the presence of free nucleotides or degraded nucleic acids. Qubit® fluorometric quantitation provides the most specific and sensitive DNA and RNA quantitation available, even at low concentrations. Transcript Dr. Amr Abid: It's hard to believe how something so simple, so light can deliver such performance. That is exactly what Qubit 2.0 does. Ashleigh Young: Qubit was a real revolution when we launched version 1.0 in 2006. We learned so much from the scientists. We listened to their suggestions and we brought them into version 2.0. With Qubit 2.0, you don't need to change yourself to fit the device. It fits you. Dr. Amr Abid: Qubit 2.0 is simply the most specific and sensitive device amongst all nucleic acid and protein quantification devices. Voice: Much time, effort, and research money is invested to obtain precious nucleic acid and protein samples. We developed Qubit 2.0 because we believe that knowing precisely and accurately the quantity of DNA, RNA or protein is so critical to the success of the downstream applications like sequencing, PCR, RT-PCR, transfection and immunoassays. Dr. Amr Abid: The device provides trust and confidence ensuring downstream applications don't suffer from inaccurate quantification. Voice: It's no surprise that these reagents and devices are now recommended by leading journals and institutions as the best for quantifying RNA or DNA in applications like q-PCR, RT-PCR and sequencing. Qubit 2.0's specificity is due to the use of different fluorophores for DNA, RNA, single-stranded DNA and protein. UV quantification method simply cannot tell or confirm if what you have in the tube is RNA, DNA, nucleotide or a mix. Basic Qubit protocol is simple and shouldn't take more than two minutes. The first step is to prepare the working solution. Use 200 microliters of buffer for each tube. Use 1 microliter of dye for each tube. Because we have five tubes in our DNA quantification assays, we will need 5 microliters of dye and 1 mL of the buffer. Mix the dye and the buffer in 1.5 mL microtubes. Now we're ready to add the standards and the DNA sample. Use thin-wall PCR tubes. For DNA quantification assay, we need two standards. The total volume in each tube should be 200 microliters. Add 190 microliters of working solution prepared previously to each tube. Add 10 microliters of the standard one in the first tube and 10 microliters of standard two in the second tube. For the DNA sample, we can use between 1 to 20 microliters of each DNA solution to an assay tube. In this case, we add 198 microliters of the working solution prepared previously in our three DNA tubes. In each of the three DNA tubes, we add 2 microliters of DNA sample and mix. We incubate the five tubes at room temperature for two minutes. We now have our two standards and three DNA samples. We're ready to measure them in the Qubit 2.0 Fluorometer. On the homescreen, you have a choice for DNA, RNA, or protein quantification. Qubit 2.0 Fluorometer has a touch screen. To choose DNA, just touch the appropriate area. You will have a choice between different ways to quantify DNA: double-stranded DNA in high-sensitive mode, double-stranded DNA in broad range mode or single-stranded DNA. In our case, we will use the broad range mode. Standard screen is automatically displayed. Insert standard number one in the sample chamber and press "Read." When the reading is done, you'll be prompted to insert standard number two and press "Read." Qubit 2.0 Fluorometer is very simple and intuitive. It will guide the user through all the measurement steps. Ashleigh Young: The Qubit 2.0 is not just a new product. It's way more than that and yet thousands and thousands of users will instantly know how to use it. Performance with simplicity. This is the very DNA of Life Technologies. Voice: Qubit fluorometric quantitation: sensitive, specific, affordable. Simply better than [using].