Stem cell research-How to use Gibco® PSC Neural Induction Medium

Generate at least 20 million NSCs from 1 million PSCs in just 7 days using Gibco® PSC Neural Induction medium. Differentiated or partially differentiated colonies should be removed before passaging. Learn how to passage and plate PSCs at the proper density and which inhibitors to use. Switching the growth medium to Gibco Neural Induction media can reach confluence within seven days. You’ll change media every other day. Colonies with a uniform morphology indicate a good neural induction. Induced NSCs can then be disassociated and expanded in neural expansion medium. Expanded NSCs can be stained with antibodies against neural markers such as Sox1, Nestin and Sox2. Expanded NSCs can also be differentiated into specific neural cell types, such as neurons, astrocytes and oligodendrocytes. Learn more at https://www.thermofisher.com/order/catalog/product/A1647801