PCR reaction types and applications

1:42

There are a number of different types of PCR reactions that can be performed, based on the polymerase used or type of data collected or needed. Standard or routine PCR relies on standard Taq polymerase and is sufficient for applications like genotyping or colony screening, which only require sequence detection. DNA polymerases may also have what are called hot-start modifications. Hot-start DNA polymerases enable room temperature reaction setup and are often preferred for PCR applications that require specific detection, such as genotyping or clinical applications. High-fidelity PCR with a proofreading enzyme is required for applications in which target DNA needs to be accurately reproduced, such as for cloning or sequencing. There are also many different types of specialty PCR. Multiplex PCR can be run on many targets within one tube, to save time, effort, and reagents. And GC-rich PCR can be performed with specific reagents developed to handle templates with high GC content, which are harder to amplify in general. There are also reagents designed for long PCR, Sometimes blends of proofreading and non- proofreading polymerases are used to synthesize long fragments.There are a number of different types of PCR reactions that can be performed, based on the polymerase used or type of data collected or needed. Standard or routine PCR relies on standard Taq polymerase and is sufficient for applications like genotyping or colony screening, which only require sequence detection. DNA polymerases may also have what are called hot-start modifications. Hot-start DNA polymerases enable room temperature reaction setup and are often preferred for PCR applications that require specific detection, such as genotyping or clinical applications. High-fidelity PCR with a proofreading enzyme is required for applications in which target DNA needs to be accurately reproduced, such as for cloning or sequencing. There are also many different types of specialty PCR. Multiplex PCR can be run on many targets within one tube, to save time, effort, and reagents. And GC-rich PCR can be performed with specific reagents developed to handle templates with high GC content, which are harder to amplify in general. There are also reagents designed for long PCR, Sometimes blends of proofreading and non- proofreading polymerases are used to synthesize long fragments.

View More
View Less

Share this video

Embed