How to achieve faster, simpler, more efficient PCR

1:17

A molecular biology method, PCR, provides efficient and specific amplification for nucleic acid analysis. After 30 PCR cycles, one copy of DNA can be amplified to hundreds of millions. Some PCR enzymes, designed for speed and high fidelity like Invitrogen Platinum SuperFi DNA Polymerase, help save significant time in cycling, as well as colony screening when used in cloning. Using a master mix that includes a dye for direct gel loading simplifies PCR protocols by helping to reduce pipetting errors, increase reproducibility, and save time in the workflow. In addition, DNA polymerases engineered for ultra-high fidelity drastically reduce the number of misincorporated nucleotides during PCR amplification. Platinum SuperFi DNA Polymerase is more than 100 times more accurate than Taq DNA polymerase. That means only one error is expected out of tens of millions of nucleotides amplified when using this high-fidelity enzyme.

View More
View Less

Share this video

Embed