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      In vitro models of human neurogenesis

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      Presented by Larisa Haupt, Queensland University of Technology The complexity of the neural niche and the ubiquitous presence of the protetoglycan proteins in the neural microenvironment suggest that this will be achievable not by one assay but will more likely be achieved through a combination of data and approaches to identify specific markers and regulatory pathways. Our approach uses human stem cell models and cell lines including human mesenchymal stem cells (hMSC), embryonic derived human neural stem cells (hNSC H9) and ENStem-A neural progenitor cells, as well as the immortalised human neural progenitors (ReNcell CX cell line), in 2D and developing 3D cultures to examine the role of proteoglycans and their use as markers of lineage potential and specification.

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