Addressing the needs of 3D suspension culture systems through the optimization of a new PSC medium

Presented by Michael Akenhead, Scientist III at Thermo Fisher Scientific Although suspension culture offers significant benefits, wider adoption has been limited due to a number of barriers. In particular, there is a lack of effective culture systems and protocols which support PSC suspension culture and scale up. To help address this gap, we have recently developed a new suspension culture media system – Gibco™ StemScale™ PSC Suspension Medium, which promotes the efficient self-aggregation of singularized PSCs into spheroids, without the need for microcarriers.