Multi-Omics and Bioinformatics in Cell Culture Media Design

"Successful media optimization depends on understanding data, specifically how each media component influences the cells. Traditionally, these data have been collected using a technique known as spent media analysis. However, the level of detail that can be obtained using spent media analysis is fundamentally restricted. This presentation will illustrate the considerable impact of applying a multi-omics analysis--utilizing proteomics and metabolomics--in obtaining a level of detail that is unparalleled in comparison to a spent media analysis approach. In particular, we will highlight how the extra level of granular intracellular detail can enable developers to either gain actionable results in less experimental iterations or, if not less iterations, gain better results from each iteration that they undertake—with the ultimate goal of making development and manufacturing more efficient and cost-effective. "