RNA Extraction from Organoids and Spheroids
Organoids and spheroids are increasingly being used in many research applications, including drug discovery, toxicology, disease modeling, and regenerative medicine. These 3D models offer an opportunity to better understand complex biology in a physiologically relevant context where 2D models have not proven as successful. Learn from our Thermo Fisher scientists about new RNA extraction methods and workflows in relation to 3D cell culture, while they give an overview of cancer innovations and applications. Directly from the bench, discover new tools used in this nucleic acid extraction for 3D cell culture. Find out best practices, as the Thermo Fisher scientists answer your questions in a Live Q&A about optimized workflows. Learning objectives: - Present 4 extraction methods to purify RNA from spheroids and organoids - Compare organic solvent and spin column kit to magnetic-bead based and cell lysate-to-signal kit, and determine the best RNA extraction for your 3D cell culture - Learn how spheroids change over time in culture For Research Use Only. Not for use in diagnostic procedures.