Streamlined TMT protocols to enable low input proteomics of freshly isolated immune cells
37:06
An easy to implement, streamlined workflow that enables quantitative proteome profiling from roughly 2 μg of protein input per experimental condition
Related Videos
In Mass Spectrometry Videos
-
Play video Measuring Protein-Turnover with Heavy Isotope Labeling and Complement Reporter Ions
Measuring Protein-Turnover with Heavy Isotope Labeling and Complement Reporter Ions
Measuring Protein-Turnover with Heavy Isotope Labeling and Complement Reporter Ions
29:34
-
Play video TMT: A Versatile and Powerful Approach for Characterization of Proteomes
TMT: A Versatile and Powerful Approach for Characterization of Proteomes
Isobaric labeling TMT experiments with optimal depth and quantitative accuracy for Proteomics.
40:02
-
Play video Enabling a High-throughput Chemo-proteomics Platform for Screening
Enabling a High-throughput Chemo-proteomics Platform for Screening
We have optimized TMT reagent usage with our platform to appropriately balance over- and under-labeling, while reducing reagent associated costs and hands on time associated with aliquoting reagents.
23:39
-
Play video Modulation of Cysteines upon cellular signalling
Modulation of Cysteines upon cellular signalling
In the present talk we will introduce cysteine modifications, various tools for assessing these and various applications were we have used cysteine-specific proteomics to characterize important cysteine residues in key proteins for cellular signallin
37:56
-
Play video Deep learning tools in PD 3.0 (CHIMERYS and INFERYS) for TMT analysis
Deep learning tools in PD 3.0 (CHIMERYS and INFERYS) for TMT analysis
Deep learning tools in PD 3.0 (CHIMERYS and INFERYS) for TMT analysis
31:42
-
Play video An Automated Sample Preparation Solution for Mass Spectrometry-based Proteomics
An Automated Sample Preparation Solution for Mass Spectrometry-based Proteomics
The automated platform enables hands-off protein lysis, DNA removal, protein reduction and alkylation, digestion, TMT labeling, pooling and cleanup, and peptide concentration measurement, providing several workflows and companion reagents to perform
18:25
