Applications of TMT-based Quantitation in Unraveling the Protein Targets of Bioactive Compounds
16:40
TMT-based thermal proteome profiling (TPP) and provide examples for identifying targets of different classes of compounds.
Related Videos
In Mass Spectrometry Videos
-
Play video An Automated Sample Preparation Solution for Mass Spectrometry-based Proteomics
An Automated Sample Preparation Solution for Mass Spectrometry-based Proteomics
The automated platform enables hands-off protein lysis, DNA removal, protein reduction and alkylation, digestion, TMT labeling, pooling and cleanup, and peptide concentration measurement, providing several workflows and companion reagents to perform
18:25
-
Play video Clinical Phosphoproteomics to Uncover New Therapeutic Strategies
Clinical Phosphoproteomics to Uncover New Therapeutic Strategies
Clinical Phosphoproteomics to Uncover New Therapeutic Strategies
26:22
-
Play video SimPLIT: Simplified sample preparation for large-scale isobaric tagging proteomics
SimPLIT: Simplified sample preparation for large-scale isobaric tagging proteomics
A simplified and cost effective one-pot reaction workflow in a 96-well plate format (SimPLIT), based on a sodium deoxycholate lysis buffer, that minimizes processing steps and demonstrates improved reproducibility compared to alternative approaches.
20:18
-
Play video Deep learning tools in PD 3.0 (CHIMERYS and INFERYS) for TMT analysis
Deep learning tools in PD 3.0 (CHIMERYS and INFERYS) for TMT analysis
Deep learning tools in PD 3.0 (CHIMERYS and INFERYS) for TMT analysis
31:42
-
Play video Enabling a High-throughput Chemo-proteomics Platform for Screening
Enabling a High-throughput Chemo-proteomics Platform for Screening
We have optimized TMT reagent usage with our platform to appropriately balance over- and under-labeling, while reducing reagent associated costs and hands on time associated with aliquoting reagents.
23:39
-
Play video Thermal Proteome Profiling for Functional and Molecular Characterization of Variant Proteins
Thermal Proteome Profiling for Functional and Molecular Characterization of Variant Proteins
We propose the use of thermal proteome profiling (TPP) for studying the impact of these disease related sequence variants on a systems wide level. TPP is a bottom up, TMT-based, quantitative mass spectrometry technique for determining the melt curves
30:52